Comparison of the development of mouse embryos manipulated with different biopsy techniques

نویسندگان

  • Ali Cihan TAŞKIN
  • Tolga AKKOÇ
  • Hakan SAĞIRKAYA
  • Haydar BAĞIŞ
  • Sezen ARAT
چکیده

Ali Cihan TAŞKIN*, Tolga AKKOÇ, Hakan SAĞIRKAYA, Haydar BAĞIŞ, Sezen ARAT College of Science, Koç University, İstanbul, Turkey Reproduction Biology Laboratory, Genetic Engineering and Biotechnology Institute, TÜBİTAK Marmara Research Center, Kocaeli, Turkey Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Uludağ University, Bursa, Turkey Department of Genetic, Faculty of Medicine, Adıyaman University, Adıyaman, Turkey Department of Biotechnology, Faculty of Agriculture, Namık Kemal University, Tekirdağ, Turkey

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

P-63: Comparison of The Efficacy of Two Culture Media (Handmade vs. Commercial) on In Vitro Development of Vitrified and Non-Vitrified Mouse Embryos

Background: While assisted reproductive techniques (ARTs) are considered to be safe, many concerns exist about the safety of these techniques. Two important and indispensable parts of these techniques are in vitro culture and vitrification. While much progress has been made in developing culture conditions, it remains suboptimal. In order to investigate the effect of culture medium on in vitro ...

متن کامل

The Evaluation of Melatonin Effect on In-Vitro Development of Mouse Preimplantation Embryos

Purpose: Melatonin promotes in-vitro embryo development in different species. This study studied the effects of melatonin on in-vitro mouse preimplantation embryo development. Materials and Methods: Two-cell embryos were obtained from oviduct of 6-8 weeks female NMRI mice 48 hours after administration of an intra-peritoneal injection of 5 IU/ml pregnant mares’ serum gonadotrophin and subsequent...

متن کامل

Comparison of the effect of Growth Factors on the Development of Pre-implantation Embryos of Vitrified Mouse

Purpose: To improve the development of frozen mouse embryos by adding fibroblast growth factors (FGF) and hepatocytes (HGF) to the culture medium. Materials and Methods: Two-cell mouse embryos obtained from the fallopian tube and frozen by cryotopic method were treated in T6 medium with a dose of 20 ng / ml FGF and 20 ng / ml HGF. The results of their culture were compared with control groups a...

متن کامل

P-23: Effect of Melatonin on In vitro Maturation, Fertilization and Resulted Embryo Development in Mouse

Background: Melatonin has been recognised as effective reactive oxygen species (ROS) scavenger. antioxident melatonin effects has been expressed in management of cancer, alzheimer, diabet diseases. This study designed to determine the effect of melatonin on in vitro oocytes maturation and fertilization and cleavage and blastocyst development rate of mouse. Materials and Methods: Cumulus - oocyt...

متن کامل

Effects of glucose and phosphate on the development of one-cell NMRI mouse embryos in M16, CZB and T6 media

  Various recent studies in both human and laboratory animals have indicated an influential role of glucose and phosphate in embryonic development of different strains and species in various media. So this study was initiated to investigate the effects of different concentrations of glucose (G0=0, Gl=0.2, G2=5.5 mM) in the absence (P0) and presence (P1=0.39 mM) of inorganic phosphate, designate...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2016